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Workshops


Monday 5 November 2018
17:00 – 18:00 h

Workshop 1
Multi-analyte test development and commercial production

Sponsored by SCIENION and Biosensing & Diagnostics (BSD), Wageningen University & Research, and presented by Dr Aart van Amerongen, BSD, Wageningen University & Research, the Netherlands and Hans Dijk, SCIENION, Germany

Scienion is a leading supplier of quality instrumentation and services for low volume liquid handling. The group BioSensing & Diagnostics (BSD) from Wageningen University & Research develops rapid, simple and/or microarray (immuno)assay formats. The workshop will give an overview of the current innovation in diagnostic testing, encompassing new, multi-analyte technologies and approaches to diagnostics with a focus on the actual test production. The workshop will cover basic test concepts in molecular diagnostics, assay design, validation, standards and QC, and an update on various assay formats and equipment (how to choose the right arrayer in your situation). SCIENION and BSD will share their vision on the future of rapid test diagnostics such as in microarraying, multi-analyte testing, automation and quantitative data analysis. There will be an opportunity to explore innovative solutions to current test manufacturing, ranging from lateral flow-based test strips to multi-analyte and miniaturised ELISA formats. The workshop objective is to discuss the practical implementation of multi-analyte tests, the evaluation of field test and laboratory use of these technologies and some sustainable solutions with respect to test cassette material. Time will be set aside for open discussion, debate and the formation of collaborations. SCIENION and BSD aim to facilitate an environment in which all involved in test manufacturing and diagnostics can learn from each other and share information and experiences.

 

Workshop 2
Real-time PCR applications for food safety

Sponsored by Bio-Rad

Bio-Rad is a leading provider of rapid methods for the detection of foodborne pathogens and quality indicator organisms. This workshop will give an overview of Bio-Rad’s complete solutions for food safety testing, focusing on real-time PCR methods that significantly reduce time to results with unparalleled sensitivity and specificity. The presentation will focus on practical applications and benefits of using real-time PCR for detection of common pathogens such as Salmonella, Listeria monocytogenes and STECand will also introduce a new PCR method for detection of Enterobacteriaceae. In addition, a novel, innovative method will also be discussed for efficient removal of free DNA in a sample without the need for toxic chemicals such a PMA. A live demonstration will be conducted to give users a chance to experience the ease of use of the method first hand and to see how real-time PCR methods can be implemented into the workflow of any laboratory, small, medium or high throughput. The workshop objective is to give a clear picture of the power of real-time PCR and its advantages in food safety diagnostics.

 


Tuesday 6 November 2018
12:45 – 13:45 h

Workshop 3
MycoKey: current developments in (multi)mycotoxin rapid screening methods

Organized by:
  • National Research Council of Italy, Institute of Sciences of Food Production, National Research Council of Italy, Italy and Ghent University, Belgium.
Supporting partner:
  • Randox Food Diagnostics, UK

The MycoKey approach on mycotoxin monitoring aims at providing straightforward, fast, timely and cost-effective solutions for growers and other stakeholders in the agro-food industry to reliably determine the amounts of mycotoxins.
The workshop will provide an overview of current developments in this field by EU and Chinese partners: :

  • a multiplex strip test for easy and rapid simultaneous determination of Fusarium toxins (fumonisins, deoxynivalenol and zearalenone) in cereals;
  • DNA aptamer-based strip tests for simultaneous analysis of aflatoxin B1 and fumonisin B1 in maize making use of quantum dots as highly sensitive labels;
  • fluorescence polarisation immunoassay for the quantitative simultaneous determination of DON, 3-acetyl-DON, 15-acetyl-DON and DON-3-glucoside in wheat;
  • fluorescence polarisation immunoassay for the quantitative simultaneous determination of T-2, HT-2, T2-glucoside and HT2-glucoside in wheat;
  • a new biosensor platform for the detection of aflatoxin B1 based on real-time electrochemical profiling; and
  • an early mycotoxin detection method based on dust sampling and analysis.
In addition, a MycoKey survey was performed for the collection and sharing of current knowledge and experience on rapid methods for mycotoxin screening. During the workshop, there will be a possibility to give a contribution to this survey. To further promote interactive discussions between scientists from public and private research involved in rapid method development, the MycoKey project established an alliance with a private company working on development of innovative methods for rapid (multi)mycotoxin detection. This work is supported by the MycoKey project “Integrated and innovative key actions for mycotoxin management in the food and feed chain” (H2020 – grant agreement no. 678781) and sponsored by Randox Food Diagnostics.

 

Workshop 4
Agile Team Diagnostics Wageningen University & Research

The Agile Team Diagnostics (ATD) from Wageningen University & Research is a collaboration of four groups that develop and apply diagnostic tests and that each belong to an application-directed contract research institute within Wageningen UR with different focus areas:

  • RIKILT Wageningen University & Research, Food Safety
  • Wageningen Plant Research, Biointeraction & Plant Health
  • Wageningen Bioveterinary Research, Animal Health and Safety
  • Wageningen Food & Biobased Research, BioSensing & Diagnostics
The team is collaborating on various detection principles (antibody-, DNA/RNA- and spectroscopy-based). The combination of Wageningen knowledge of animals, food, plants, agrotechnology, environment and water, together with the broad expertise on various diagnostic platforms, opens up unique opportunities to innovative solutions. In addition to development of new diagnostics the ATD is also offering services to governmental bodies and companies. At the workshop a short introduction on the Agile Team Diagnostics will be given followed by a number of demonstrations that will show the diversity of diagnostic platforms and applications being addressed in Wageningen.